$<$h2$>$Abstract$<$/h2$><$h3$>$Purpose$<$/h3$><$p$>$Temporal lobe epilepsy is associated with tissue abnormalities of several gray and white matter structures that are reproduced in animal models. Few longitudinal studies have focused on the identification of structural differences during epileptogenesis. The diffusion tensor model is a useful tool for evaluating cell death, gliosis, and axonal plasticity in epileptic subjects. This study aimed to evaluate temporal tissue changes after experimental emphstatus epilepticus in an animal model of chronic temporal lobe epilepsy.$<$/p$><$h3$>$Methods$<$/h3$><$p$>$Systemic pilocarpine-induced emphstatus epilepticus in adult Sprague-Dawley rats. Animals were scanned using diffusion tensor imaging (DTI) at three time points: prior to emphstatus epilepticus, and 24 and 64 days post-induction (early and late chronic, respectively). Fractional anisotropy, apparent diffusion coefficient, axial diffusivity (Dtextsubscript|), and radial diffusivity (Dtextsubscript+) were evaluated in white (fimbria, cingulum, corpus callosum, and internal capsule) and gray (dorsal hippocampus, dentate gyrus, and CA3) matter regions for the three time points. Histological assessment of neurodegeneration in Klüver-Barrera preparations from the same animals was performed.$<$/p$><$h3$>$Results$<$/h3$><$p$>$Significantly reduced volume of dorsal hippocampus and fimbria of the epileptic animals was observed already at 24 days post-emphstatus epilepticus. Progressive changes of DTI parameters in both the white and gray matter structures of the experimental group were also observed. Stained sections confirmed such alterations.$<$/p$><$h3$>$Conclusion$<$/h3$><$p$>$Our study revealed time-dependent diffusion changes in gray and white matter structures after pilocarpine-induced emphstatus epilepticus. The characterization of these alterations over time may be potential imaging markers for epileptogenesis.$<$/p$>$